Powder: -20°C for 3 years | In solvent: -80°C for 1 year
VO-Ohpic trihydrate (VO-Ohpic) 是一种有效的磷酸酶和张力蛋白同源物(PTEN)抑制剂,IC50 为 35 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 219 | 现货 | ||
5 mg | ¥ 488 | 现货 | ||
10 mg | ¥ 763 | 现货 | ||
25 mg | ¥ 1,360 | 现货 | ||
50 mg | ¥ 2,270 | 现货 | ||
100 mg | ¥ 3,730 | 现货 | ||
200 mg | ¥ 5,420 | 现货 | ||
500 mg | ¥ 8,380 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 488 | 现货 |
产品描述 | VO-Ohpic trihydrate (VO-Ohpic) is a potent inhibitor of PTEN (phosphatase and tensin homolog) with IC50 of 35 nM. |
靶点活性 | PTEN:35 nM |
体外活性 | VO-OHpic is a potent small-molecule compound that specifically inhibits PTEN's cellular enzymatic activity, which in turn activates downstream targets such as Akt and FoxO3a. Glucose uptake into adipocytes is dramatically enhanced upon PTEN inhibition with VO-OHpic. PTEN inhibitor accelerates wound healing in fibroblasts[1]. VO-OHpic inhibits cell viability, cell proliferation and colony formation, and induces senescence-associated β-galactosidase activity in Hep3B (low PTEN expression) and to a lesser extent in PLC/PRF/5 (high PTEN expression) cells, but not in PTEN-negative SNU475 cells[2]. |
体内活性 | VO-Ohpic significantly inhibits tumor growth in nude mice bearing xenografts of Hep3B cells[2]. VO-Ohpic administered to C57BL6 mice 30 minutes prior to Kcl-induced asystolic cardiac arrest significantly increases survival, LVPmax and dP/dt max with continued benefit. VO-OHpic also significantly increases lactate clearance and decreases plasma glucose level[3]. |
激酶实验 | VO-OHpic is dissolved in DMSO (100 μM) and diluted further to the required concentration with 1% DMSO. For inhibition studies, PTEN is preincubated with VO-OHpic at RT for 10 min before substrate is added to initialise the reaction. Background absorbance (malachite green assay) and fluorescence (OMFP assay) are determined with VO-OHpic in assay buffer and corrected in the data analysis[1]. |
细胞实验 | Cell proliferation is determined by estimating the amount of bromodeoxyuridine (BrdU) incorporation into DNA by a colorimetric immunoassay. 3×103 cells are cultured in 96-well plates with varying concentrations of VO-OHpic for 72 hours. BrdU is added 24 hours before the end of the treatments. Results are expressed as the percentage inhibition of BrdU incorporation over the control.(Only for Reference) |
别名 | VO-Ohpic |
分子量 | 415.2 |
分子式 | C12H9N2O8V·3H2O·H |
CAS No. | 476310-60-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 4.15 mg/mL (10 mM), Sonication is recommended.
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.4085 mL | 12.0424 mL | 24.0848 mL | 60.2119 mL |
5 mM | 0.4817 mL | 2.4085 mL | 4.817 mL | 12.0424 mL | |
10 mM | 0.2408 mL | 1.2042 mL | 2.4085 mL | 6.0212 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
VO-Ohpic trihydrate 476310-60-8 Autophagy PI3K/Akt/mTOR signaling PTEN Inhibitor VO-Ohpic VOOhpic trihydrate VO Ohpic trihydrate inhibit VO-Ohpic Trihydrate Phosphatase and tensin homolog MMAC1 inhibitor